Journal: Frontiers in Immunology
Article Title: Effect of hypoxia-induced mIL15 expression on expansion and memory progenitor stem-like TILs in vitro
doi: 10.3389/fimmu.2024.1450245
Figure Lengend Snippet: Generation and identification of mIL15-expressing TILs. (A) Schematic diagram of lentiviral vector encoding mIL15. (B) Representative flow cytometry plot of mIL15 + cells at 5 d after pre-REP TIL transduction. (C) Statistical plot showing the percentage of mIL15 + cells (n=3). (D) ELISA analysis of IL15 secretion in culture supernatants of UN-TIL+IL2 and TIL-mIL15+IL2 cells (n=3). Statistical analysis performed using two-tailed unpaired Student’s t-test. Statistical significance defined as: **P<0.01; ***P<0.001.
Article Snippet: On April 22, 2024, the IL15 super-agonist N-803 (Anktiva) developed by ImmunityBio was approved by the FDA for use in combination with BCG to treat BCG-unresponsive non-muscle invasive bladder cancer with carcinoma in situ .
Techniques: Expressing, Plasmid Preparation, Flow Cytometry, Transduction, Enzyme-linked Immunosorbent Assay, Two Tailed Test